VERSATILE
available in Widefield, Spinning-disk confocal, and TIRF configurations
AFFORDABLE
the most cost-effective way of doing quantitative FLIM
COMPATIBLE
LIFA is compatible with every fluorescence microscope with a camera output
The Lambert Instruments LIFA system is the fastest and easiest way to do Fluorescence Lifetime Imaging Microscopy (FLIM).
Available in versatile configurations dependent on your specific applications, the LIFA system offers a turn-key solution for fluorescence lifetime imaging microscopy. Compatible with every fluorescence microscope with a camera output - including microscopes by Leica, Nikon, Olympus, TILL and Zeiss; set up is easy and fast, with all hardware integrating seamlessly with our LIFA software, so you can focus on your experiment.
The advanced software instantly analyses your data and presents the calculated fluorescence lifetimes visually. Recorded images are compatible with ImageJ, FIJI, Matlab and MetaMorph, while detailed statisitcal data can be exported to Excel worksheets.
Read more about LIFA software.
LIFA vTAU
SPAD powered FLIM system
Simplify experiments for researchers and imaging centres with the new vTAU SPAD camera; combining excellent light sensitivity with easy image acquisition and data analysis.
Minimise measurement duration, automate image acquisition, and simplify data analysis... factors of great importance for cell biology, cancer research and high-throughput screening.
vTAU easily integrates into any FLIM system, providing a plug-n-play experience that allows for switching between setups.
Key features
Unique image sensor
Ultra-high sensitive SPAD detector for up to 100 lifetime images per second
Mulitple image modes
Including regular Frequency-Domain FLIM acquisition and Time-Lapse recordings
Characteristics
- Pixel resolution: 512 x 512 px
- Pixel size: 16 μm
- Lifetime range: 0.2 to 300ns
- Frame rate: up to 300 fps
- Sensor type: SPAD
LIFA with TRiCAM
Time-domain FLIM for Widefield microscopes
With a gated TRiCAM camera, LIFA records a series of images and automatically shifts the timing between the light pulse and the camera exposure.
Fluorescence lifetime can be recorded for every pixel in the image simultaneously, and the image data can then be exported to popular imaging software for further analysis.
Typically more cost-effective than methods that require a confocal set-up, the LIFA with TRiCAM standard configuration offers an entry-level setup for FLIM measurements.
Key features
Accurate lifetime determination
Fluorescence lifetimes from 0-300 ns with an accuracy of 30 ps r.m.s.
High resolution image intensifier
Highest resolution and sensitivity in the UV, visible or near infrared.
Characteristics
- Pixel resolution: 1920 x 1200 px
- Pixel size: 7.6 μm
- Typical Dark current: <7 e-/s
- Readout noise: <6.5 e- (subject to change)
- Framerate: >100 fps
Featured LIFA users
